Confirmed identities of proteins from a two-dimensional map of Syrian hamster embryo cells.

The effects of specific antibody-complement-mediated cytotoxicity on transformed and untransformed Syrian hamster cells.

Antibodies were produced against 220,000-molecular-weight proteins of Syrian hamster embryo cells. The antiserum containing these antibodies is capable of immunologically staining the surfaces and a fibrillar network around untransformed Syrian hamster embryo cells. The staining is removed by adsorption of the antiserum with Syrian hamster embryo cells and by mild trypsin treatment. Several lin...

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis

Changes in gene expression accompanying neoplastic transformation of Syrian hamster cells.

Proteins solubilized from the chemically transformed, highly tumorigenic Syrian hamster cell line, BP6T, and the untransformed parental embryo cells, have been analyzed by two-dimensional gel electrophoresis. Differences in seven major polypeptides have been identified in cytoplasmic and nuclear cell fractions from these two related cell types. The tumorigenic cells have lost the ability to syn...

Spontaneous differentiated squamous cell carcinoma of cheek pouch in a Syrian hamster

A 2-year-old male hamster was presented because of left facial swelling and salivation. Necropsy wasperformed and affected cheek pouch with tissue samples of regional and internal organs were submitted forhistopathological study. In histopathological examination it was found that the tumor consisted of cords andislands of tumoric epithelial cells which were diffused in the dermis. Vacoulation o...

Selective reduction in receptors for epidermal growth factor-urogastrone in chemically transformed tumorigenic Syrian hamster embryo fibroblasts.

Receptors for insulin and epidermal growth factor-urogastrone (EGF-URO) have been measured in Syrian hamster em bryo fibroblasts and in a benzo(a)pyrene-transformed tumorigenie cell line (BP6T). Compared with the parent Syrian ham ster embryo cells, the BP6T cells exhibit a marked reduction in the binding of EGF-URO, whereas the binding of insulin is the same in both cell types. The selective r...